江苏省科技期刊可视化阅读平台

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江苏科技信息 ›› 2015, Vol. 32 ›› Issue (7): 65-66,77.doi: 10.3969/j.issn.1004-7530.2015.07.016

• 论文 • 上一篇    下一篇

结核分枝杆菌HspX基因真核表达载体构建

髙莉莉, 徐苏娟, 张继明, 王建杰   

  1. 佳木斯大学，黑龙江佳木斯 154002; 牡丹江医学院红旗医院检验科，黑龙江牡丹江 157011;彩虹医院,陕西咸阳,712021;佳木斯大学,黑龙江佳木斯,154002
  • 出版日期:2015-03-05 发布日期:2015-03-05

Construction of Eukaryotic Expression Vector of Mycobacterium Tuberculosis HspX Gene

Gao Lili, Xu Sujuan, Zhang Jiming, Wang Jianjie   

  • Online:2015-03-05 Published:2015-03-05

摘要： 为构建结核分枝杆菌HspX基因与增强型绿色荧光蛋白（EGFP）融合基因的真核表达载体，文章采用PCR法扩增HspX基因，定向插入到质粒pEGFP-N1的多克隆位点。结果显示，融合基因真核表达载体，经双酶切鉴定、基因测序鉴定克隆片段大小和序列正确。文章成功构建了pEGFP-N1-HspX融合基因真核表达载体。

关键词: 结核分枝杆菌, HspX, 真核表达

Abstract: In order to construct a recombinant plasmid carrying enhanced green fluorescent protein (EGFP)and Mycobacterium tuberculosis HspX gene,HspX gene was amplified by PCR and inserted into a multiple cloning site of eukaryotic expression vector pEGFP-N1. The results shows HspX eukaryotic expression vector labeled with FLAG tag was successfully constructed as demonstrated by double enzyme digestion and DNA sequencing. The conclusion is that the eukaryotic expression plasmid pEGFP-N1-HspX has been constructed successfully.